FIg. 1. DHA is enriched in the glyceride fraction efficiently, since Candida rugosa
lipase acts extremely weakly on DHA. DHA concentration can only increase up to a
certain point no matter how much lipase is dosed. Re-hydrolysis of DHA-rich oil fraction separated from the reaction mixture achieves higher concentrations of DHA.
FIg. 2. the rearrangement of pUFAs esterified and interesterified by Candida rugosa
lipase provides di-pUFA tAg and/or tri-pUFA tAg. Hydrolysis kinetics of tAg depend
on the number of pUFA bound in glycerol moiety.